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cd45 hi30  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences cd45 hi30
    Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, <t>CD45,</t> and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.
    Cd45 Hi30, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+hi30/APC-Cyanine7+Anti-Human+CD45/pmc12856185-108-0-4
    Average 94 stars, based on 1 article reviews
    cd45 hi30 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Protocol for morphogen-guided differentiation of brain cell types using human induced pluripotent stem cells"

    Article Title: Protocol for morphogen-guided differentiation of brain cell types using human induced pluripotent stem cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.104339

    Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, CD45, and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.
    Figure Legend Snippet: Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, CD45, and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.

    Techniques Used: Flow Cytometry, Staining, Expressing



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    cd45 hi30  (Cytek Biosciences)
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    Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, <t>CD45,</t> and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.
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    Image Search Results


    Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, CD45, and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.

    Journal: STAR Protocols

    Article Title: Protocol for morphogen-guided differentiation of brain cell types using human induced pluripotent stem cells

    doi: 10.1016/j.xpro.2025.104339

    Figure Lengend Snippet: Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, CD45, and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.

    Article Snippet: CD45 (HI30) (1:50) , Tonbo Bioscience , Cat# 25-0459, RRID: AB_2621631.

    Techniques: Flow Cytometry, Staining, Expressing